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KMID : 0370220150590010006
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Yakhak Hoeji
2015 Volume.59 No. 1 p.6 ~ p.11
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A Simple HPLC-UV Method for the Quantification of Isepamicin in Human Serum: Pharmacokinetic Applications
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Hwang Eun-Kyung
Jeon Seong-Sill
Shin Young-Hee
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Abstract
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A simple HPLC-UV method was developed and validated for the quantification of human serum isepamicin using phenylisocyanate as a derivatization agent. The linear calibration curve was obtained in the concentration range of 1~100 μg/ml and LLOQ of 1 μg/ml. This method was validated with selectivity, linearity, precision and accuracy, leading to successful application for estimating the pharmacokinetic parameters of isepamicin in Korean healthy subjects following IV infusion of 800 mg isepamicin. The mean t1/2 of isepamicin was 1.55±0.08 hr.
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KEYWORD
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isepamicin, phenylisocyanate, derivatization, HPLC-UV, human serum, pharmacokinetics
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